Ever since chemists Started using separating funnels to isolate chemicals by partitioning, they have understood the potential advantages of liquid or liquid chromatography, known now as countercurrent chromatography CCC. Until recently, CCC was primarily a technique for organic products or academic study and has been barely used in mainstream purification. Unfortunately, ancient CCC instrumentation was badly engineered and suffered from slow pace of separation, a combination that resulted in slight adoption as a complementary and orthogonal chromatography technique. However a new generation of high-performance countercurrent chromatography HPCCC instrumentation has resulted in the rebirth of liquid or liquid chromatography in the 21st century and for that reason offering a greater advantage to the chemists. CCC can significantly enhance a chemists productivity and different compounds which were previously very hard to isolate or uneconomical to produce.
Moreover, since both stationary and mobile phases are fluids, we gain two additional important productivity benefits. The advantages of HPCCC into chemists not only boosts the productivity in any way scales it is also a technique which can be implemented across the entire variety of polarity and to both small and large synthetic molecules, peptides, and organic products. Since HPCCC is a high-capacity technique, it is becoming the first choice for scientists when they should generate huge amounts of target compounds. This is particularly attractive when a chemical and its analogues are recognized as a lead candidate involved in ever-increasing quantities as they advance throughout the pharmaceutical development process. Using HPCCC instruments chemists have the ability to focus on their product development process, not purification or chromatography redevelopment, as scale increases.
what is a chromatogram Another productivity advantage is that with CCC There is absolutely not any chance of irreversible adsorption happening either onto or in the stationary phase. Recoveries are always very high, and it is certain that the whole sample will elute from the column. Performing scale up of purification involving different sizes of HPCCC tools is fast and simple. One simply uses the volumetric ratio between the two column volumes one wants to use to ascertain the new sample volume and cellular phase follow speed. A Further significant advantage concerns sample solubility. As opposed to the solubility of samples becoming a limiting factor, they tend toward irrelevance because the sample can be injected onto the column in either the mobile, stationary, or a mix of both stages, without affecting the functioning of the chromatography. It is possible with pumps that operate at 50mL or min to easily process up to 200g of primitive material each day and possibly up to 400g. This is a substantial progress in reducing the chromatography bottleneck resulting from the throughput limitations of liquid or solid chromatography methods or the solubility of samples. HPCCC can help resolve these issues.